TB Genome Annotation Portal

Rv1086 (-)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: short-chain Z-isoprenyl diphosphate synthase
  • REFSEQ: short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase)
  • PATRIC: Undecaprenyl pyrophosphate synthetase (EC 2.5.1.31)
  • TUBERCULIST: Short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase)
  • NCBI: Short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase)
  • updated information (H37Rv4):
    • gene name: -
    • function: E,Z-farnesyl diphosphate synthase
    • reference: Wang 2008, PMID: 18597781
• Type: Essential
• Start: 1210595
• End: 1211383
• Operon:


• Trans-membrane region:
  
• Role: V - Conserved hypotheticals
• GO terms:
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0033850 - Z-farnesyl diphosphate synthase activity (Uniprot)
  • GO:0030145 - manganese ion binding (Uniprot)
  • GO:0016765 - transferase activity, transferring alkyl or aryl (other than methyl) groups (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0016094 - polyprenol biosynthetic process (Uniprot)
  • GO:0016020 - membrane (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0002094 - polyprenyltransferase activity (Uniprot)
  • GO:0000287 - magnesium ion binding (Uniprot)
  • GO:0016042 - lipid catabolic process (FLUTE) (FLUTE Assigned!)
  • GO:0009252 - peptidoglycan biosynthetic process (FLUTE) (FLUTE Assigned!Y2 report, Kieser et al.)
• Reaction(s) (based on iSM810 metabolic model):
  • IPP[c] + GPP[c] -> PPI[c] + FPP[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 2vg0, 2vfw, 2vg1
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  2VG1	100%	MYCOBACTERIUM TUBERCULOSIS	Rv1086 E,E-farnesyl diphosphate complex
  2VG0	100%	MYCOBACTERIUM TUBERCULOSIS	Rv1086 citronellyl pyrophosphate complex
  2VFW	100%	MYCOBACTERIUM TUBERCULOSIS	Rv1086 native
  7CPN	36%	Thermobifida fusca (strain YX)	CRYSTAL STRUCTURE OF DODECAPRENYL DIPHOSPHATE SYNTHASE FROM THERMOBIFIDA FUSCA
  7CPM	36%	Thermobifida fusca (strain YX)	CRYSTAL STRUCTURE OF DODECAPRENYL DIPHOSPHATE SYNTHASE FROM THERMOBIFIDA FUSCA
  6IME	35%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Rv2361c complex with substrate analogues
  4ONC	35%	Mycobacterium tuberculosis	Crystal Structure of Mycobacterium Tuberculosis Decaprenyl Diphosphate Synthase in Complex with BPH-640
  2VG4	35%	MYCOBACTERIUM TUBERCULOSIS	Rv2361 native
  2VG3	35%	MYCOBACTERIUM TUBERCULOSIS	Rv2361 with citronellyl pyrophosphate
  2VG2	35%	MYCOBACTERIUM TUBERCULOSIS	Rv2361 with IPP

• Links to additional information on Rv1086:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -0.718 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VEIIPPRLKEPLYRLYELRLRQGLAASKSDLPRHIAVLCDGNRRWARSAGYDDVSYGYRMGAAKIAEMLRWCHEAGIELATVYLLSTENLQRDPDELAAL
IEIITDVVEEICAPANHWSVRTVGDLGLIGEEPARRLRGAVESTPEVASFHVNVAVGYGGRREIVDAVRALLSKELANGATAEELVDAVTVEGISENLYT
SGQPDPDLVIRTSGEQRLSGFLLWQSAYSEMWFTEAHWPAFRHVDFLRALRDYSARHRSYGR

Additional Information