Rv1049 (-)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: transcriptional repressor
- REFSEQ: transcriptional repressor protein
- PATRIC: Organic hydroperoxide resistance transcriptional regulator
- TUBERCULIST: Probable transcriptional repressor protein
- NCBI: Probable transcriptional repressor protein
- updated information (H37Rv4):
  - gene name: mosR2
  - function: oxidation-sensing transcription factor
  - reference: Brugarolas (2017) PMID: 22992749
Type: Not Target
Start: 1172386
End: 1172832
Operon:

Trans-membrane region:
Role: I.J.1 - Repressors/activators
GO terms:
- GO:0044212 - transcription regulatory region DNA binding (Uniprot)
- GO:0006355 - regulation of transcription, DNA-templated (Uniprot)
- GO:0003700 - DNA binding transcription factor activity (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure: 4fx0, 4fx4
Search for Homologs in PDB

Top 10 Homologs in PDB (as of Nov 2020):

PDB	aa ident	species	PDB title
4FX4	100%	Mycobacterium tuberculosis	Crystal structure of M. tuberculosis transcriptional regulator MOSR (Rv1049) in compex with DNA
4FX0	100%	Mycobacterium tuberculosis	Crystal structure of M. tuberculosis transcriptional regulator MosR

Links to additional information on Rv1049:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.332 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

MGKGAAFDECACYTTRRAARQLGQAYDRALRPSGLTNTQFSTLAVISLSEGSAGIDLTMSELAARIGVERTTLTRNLEVMRRDGLVRVMAGADARCKRIE
LTAKGRAALQKAVPLWRGVQAEVTASVGDWPRVRRDIANLGQAAEACR

(Nucleotide sequence available on KEGG)

Additional Information

oxidation-sensing transcription factor (like dosR and Rv0081).
more specific to NO (nitric oxide) response.

Cortes T, Schubert OT, Banaei-Esfahani A, Collins BC, Aebersold R,
Young DB. Delayed effects of transcriptional responses in
Mycobacterium tuberculosis exposed to nitric oxide suggest other
mechanisms involved in survival. Sci Rep. 2017 Aug
15;7(1):8208. doi: 10.1038/s41598-017-08306-1. PMID: 28811595;
PMCID: PMC5557973.

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Non-Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 2 non-insertions in a row out of 4 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 4 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 4 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 0 non-insertions in a row out of 4 sites
Non-Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 0 non-insertions in a row out of 4 sites
Non-Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Non-Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 0.69
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- Rv0006 (gyrA) (Direct binding)
- Rv0019c (fhaB) (Direct binding)
- Rv0032 (bioF2) (Direct binding)
- Rv0057 (-) (Direct binding)
- Rv0058 (dnaB) (Direct binding)
- Rv0107c (ctpI) (Direct binding)
- Rv0134 (ephF) (Direct binding)
- Rv0144 (-) (Direct binding)
- Rv0145 (-) (Direct binding)
- Rv0156 (pntAb) (Direct binding)
- Rv0167 (yrbE1A) (Direct binding)
- Rv0175 (-) (Direct binding)
- Rv0210 (-) (Direct binding)
- Rv0242c (fabG4) (Direct binding)
- Rv0243 (fadA2) (Direct binding)
- Rv0247c (-) (Direct binding)
- Rv0308 (-) (Direct binding)
- Rv0344c (lpqJ) (Direct binding)
- Rv0345 (-) (Direct binding)
- Rv0354c (PPE7) (Direct binding)
- Rv0425c (ctpH) (Direct binding)
- Rv0451c (mmpS4) (Direct binding)
- Rv0452 (-) (Direct binding)
- Rv0454 (-) (Direct binding)
- Rv0470c (pcaA) (Direct binding)
- Rv0476 (-) (Direct binding)
- Rv0483 (lprQ) (Direct binding)
- Rv0512 (hemB) (Direct binding)
- Rv0522 (gabP) (Direct binding)
- Rv0549c (vapC3) (Direct binding)
- Rv0550c (vapB3) (Direct binding)
- Rv0636 (hadB) (Direct binding)
- Rv0643c (mmaA3) (Direct binding)
- Rv0649 (fabD2) (Direct binding)
- Rv0685 (tuf) (Direct binding)
- Rv0688 (-) (Direct binding)
- Rv0702 (rplD) (Direct binding)
- Rv0706 (rplV) (Direct binding)
- Rv0730 (-) (Direct binding)
- Rv0808 (purF) (Direct binding)
- Rv0809 (purM) (Direct binding)
- Rv0824c (desA1) (Direct binding)
- Rv0983 (pepD) (Direct binding)
- Rv1049 (-) (Direct binding)
- Rv1050 (-) (Direct binding)
- Rv1075c (-) (Direct binding)
- Rv1089A (celA2a) (Direct binding)
- Rv1094 (desA2) (Direct binding)
- Rv1146 (mmpL13b) (Direct binding)
- Rv1177 (fdxC) (Direct binding)
- Rv1208 (gpgS) (Direct binding)
- Rv1212c (glgA) (Direct binding)
- Rv1277 (-) (Direct binding)
- Rv1336 (cysM) (Direct binding)
- Rv1498A (-) (Direct binding)
- Rv1499 (-) (Direct binding)
- Rv1566c (-) (Direct binding)
- Rv1586c (-) (Direct binding)
- Rv1587c (-) (Direct binding)
- Rv1639c (-) (Direct binding)
- Rv1655 (argD) (Direct binding)
- Rv1780 (-) (Direct binding)
- Rv1796 (mycP5) (Direct binding)
- Rv1848 (ureA) (Direct binding)
- Rv1861 (-) (Direct binding)
- Rv1901 (cinA) (Direct binding)
- Rv1985c (-) (Direct binding)
- Rv1986 (-) (Direct binding)
- Rv2052c (-) (Direct binding)
- Rv2067c (-) (Direct binding)
- Rv2107 (PE22) (Direct binding)
- Rv2134c (-) (Direct binding)
- Rv2139 (pyrD) (Direct binding)
- Rv2146c (-) (Direct binding)
- Rv2147c (-) (Direct binding)
- Rv2148c (-) (Direct binding)
- Rv2224c (caeA) (Direct binding)
- Rv2238c (ahpE) (Direct binding)
- Rv2239c (-) (Direct binding)
- Rv2256c (-) (Direct binding)
- Rv2271 (-) (Direct binding)
- Rv2438A (-) (Direct binding)
- Rv2438c (nadE) (Direct binding)
- Rv2442c (rplU) (Direct binding)
- Rv2456c (-) (Direct binding)
- Rv2546 (vapC18) (Direct binding)
- Rv2645 (-) (Direct binding)
- Rv2720 (lexA) (Direct binding)
- Rv2737A (-) (Direct binding)
- Rv2767c (-) (Direct binding)
- Rv2783c (gpsI) (Direct binding)
- Rv2838c (rbfA) (Direct binding)
- Rv2905 (lppW) (Direct binding)
- Rv2926c (-) (Direct binding)
- Rv2934 (ppsD) (Direct binding)
- Rv2935 (ppsE) (Direct binding)
- Rv2941 (fadD28) (Direct binding)
- Rv2953 (-) (Direct binding)
- Rv2988c (leuC) (Direct binding)
- Rv2989 (-) (Direct binding)
- Rv3072c (-) (Direct binding)
- Rv3105c (prfB) (Direct binding)
- Rv3106 (fprA) (Direct binding)
- Rv3158 (nuoN) (Direct binding)
- Rv3312c (-) (Direct binding)
- Rv3359 (-) (Direct binding)
- Rv3360 (-) (Direct binding)
- Rv3364c (-) (Direct binding)
- Rv3477 (PE31) (Direct binding)
- Rv3580c (cysS1) (Direct binding)
- Rv3607c (folB) (Direct binding)
- Rv3610c (ftsH) (Direct binding)
- Rv3611 (-) (Direct binding)
- Rv3616c (espA) (Direct binding)
- Rv3624c (hpt) (Direct binding)
- Rv3660c (-) (Direct binding)
- Rv3673c (-) (Direct binding)
- Rv3714c (-) (Direct binding)
- Rv3719 (-) (Direct binding)
- Rv3750c (-) (Direct binding)
- Rv3803c (fbpD) (Direct binding)
- Rv3849 (espR) (Direct binding)
- Rv3862c (whiB6) (Direct binding)
- Rv3873 (PPE68) (Direct binding)
- Rv3878 (espJ) (Direct binding)
- Rv0033 (acpA) (Upstream of operon)
- Rv0034 (-) (Upstream of operon)
- Rv0035 (fadD34) (Upstream of operon)
- Rv0168 (yrbE1B) (Upstream of operon)
- Rv0169 (mce1A) (Upstream of operon)
- Rv0170 (mce1B) (Upstream of operon)
- Rv0171 (mce1C) (Upstream of operon)
- Rv0172 (mce1D) (Upstream of operon)
- Rv0173 (lprK) (Upstream of operon)
- Rv0174 (mce1F) (Upstream of operon)
- Rv0176 (-) (Upstream of operon)
- Rv0177 (-) (Upstream of operon)
- Rv0178 (-) (Upstream of operon)
- Rv0241c (htdX) (Upstream of operon)
- Rv0449c (-) (Upstream of operon)
- Rv0450c (mmpL4) (Upstream of operon)
- Rv0477 (-) (Upstream of operon)
- Rv0478 (deoC) (Upstream of operon)
- Rv0513 (-) (Upstream of operon)
- Rv0514 (-) (Upstream of operon)
- Rv0984 (moaB2) (Upstream of operon)
- Rv1090 (celA2b) (Upstream of operon)
- Rv1178 (-) (Upstream of operon)
- Rv1278 (-) (Upstream of operon)
- Rv1279 (-) (Upstream of operon)
- Rv1849 (ureB) (Upstream of operon)
- Rv1850 (ureC) (Upstream of operon)
- Rv1851 (ureF) (Upstream of operon)
- Rv1852 (ureG) (Upstream of operon)
- Rv1853 (ureD) (Upstream of operon)
- Rv2133c (-) (Upstream of operon)
- Rv2441c (rpmA) (Upstream of operon)
- Rv2547 (vapB19) (Upstream of operon)
- Rv2548 (vapC19) (Upstream of operon)
- Rv2646 (-) (Upstream of operon)
- Rv2766c (-) (Upstream of operon)
- Rv2782c (pepR) (Upstream of operon)
- Rv2925c (rnc) (Upstream of operon)
- Rv2942 (mmpL7) (Upstream of operon)
- Rv2987c (leuD) (Upstream of operon)
- Rv3103c (-) (Upstream of operon)
- Rv3104c (-) (Upstream of operon)
- Rv3478 (PPE60) (Upstream of operon)
- Rv3579c (-) (Upstream of operon)
- Rv3605c (-) (Upstream of operon)
- Rv3606c (folK) (Upstream of operon)
- Rv3608c (folP1) (Upstream of operon)
- Rv3609c (folE) (Upstream of operon)
- Rv3720 (-) (Upstream of operon)
Bound By:
- Rv1049 (-) (Direct binding)

TB Genome Annotation Portal

Rv1049 (-)

Amino Acid Sequence

Additional Information

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

Interactions based on ChIPSeq data

Interactions based on ChIPSeq data (Minch et al. 2014)

Interactions based on TFOE data (Rustad et al. 2014)

TBCAP

Comments