TB Genome Annotation Portal

Rv0886 (fprB)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: ferredoxin-NADP+ reductase
  • REFSEQ: NADPH:adrenodoxin oxidoreductase FprB
  • PATRIC: Ferredoxin / Ferredoxin--NADP(+) reductase actinobacterial (eukaryote-like) type (EC 1.18.1.2)
  • TUBERCULIST: Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase)
  • NCBI: Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase)
  • updated information (H37Rv4):
    • gene name: fprB
    • function:
    • reference:
• Type: Not Target
• Start: 983803
• End: 985530
• Operon:


• Trans-membrane region:
  
• Role: I.B.6.c - Electron transport
• GO terms:
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0051539 - 4 iron, 4 sulfur cluster binding (Uniprot)
  • GO:0051536 - iron-sulfur cluster binding (Uniprot)
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0004324 - ferredoxin-NADP+ reductase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • NADP[c] + 2 FERO[c] <=> NADPH[c] + 2 FERI[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  2VKR	48%	ACIDIANUS AMBIVALENS	3Fe-4S, 4Fe-4S plus Zn Acidianus ambivalens ferredoxin
  1H98	43%	THERMUS AQUATICUS	New Insights into Thermostability of Bacterial Ferredoxins: High Resolution Crystal Structure of the Seven-Iron Ferredoxin from Thermus thermophilus
  5T61	41%	Methanothermobacter wolfeii	TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOTHERMOBACTER WOLFEII, TRICLINIC FORM AT 2.55 A
  5T5M	41%	Methanothermobacter wolfeii	TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOTHERMOBACTER WOLFEII, TRIGONAL FORM AT 2.5 A.
  5T5I	41%	Methanothermobacter wolfeii	TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOTHERMOBACTER WOLFEII, ORTHORHOMBIC FORM AT 1.9 A
  1BD6	41%	Bacillus schlegelii	7-FE FERREDOXIN FROM BACILLUS SCHLEGELII, NMR, MINIMIZED AVERAGE STRUCTURE
  1BC6	41%	Bacillus schlegelii	7-FE FERREDOXIN FROM BACILLUS SCHLEGELII, NMR, 20 STRUCTURES
  1LQU	41%	Mycobacterium tuberculosis	Mycobacterium tuberculosis FprA in complex with NADPH
  1LQT	41%	Mycobacterium tuberculosis	A covalent modification of NADP+ revealed by the atomic resolution structure of FprA, a Mycobacterium tuberculosis oxidoreductase
  2C7G	40%	MYCOBACTERIUM TUBERCULOSIS	FprA from Mycobacterium tuberculosis: His57Gln mutant

• Links to additional information on fprB:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.902 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MPHVITQSCCNDASCVFACPVNCIHPTPDEPGFATSEMLYIDPVACVDCGACVTACPVSAIAPNTRLDFEQLPFVEINASYYPKRPAGVKLAPTSKLAPV
TPAAEVRVRRQPLTVAVVGSGPAAMYAADELLVQQGVQVNVFEKLPTPYGLVRSGVAPDHQNTKRVTRLFDRIAGHRRFRFYLNVEIGKHLGHAELLAHH
HAVLYAVGAPDDRRLTIDGMGLPGTGTATELVAWLNGHPDFNDLPVDLSHERVVIIGNGNVALDVARVLAADPHELAATDIADHALSALRNSAVREVVVA
ARRGPAHSAFTLPELIGLTAGADVVLDPGDHQRVLDDLAIVADPLTRNKLEILSTLGDGSAPARRVGRPRIRLAYRLTPRRVLGQRRAGGVQFSVTGTDE
LRQLDAGLVLTSIGYRGKPIPDLPFDEQAALVPNDGGRVIDPGTGEPVPGAYVAGWIKRGPTGFIGTNKSCSMQTVQALVADFNDGRLTDPVATPTALDQ
LVQARQPQAIGCAGWRAIDAAEIARGSADGRVRNKFTDVAEMLAAATSAPKEPLRRRVLARLRDLGQPIVLTVPL

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	2.47 (0.69)	1.53 (0.66)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link