Rv0845 (-)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: two-component system NarL family sensor kinase
- REFSEQ: two component sensor kinase
- PATRIC: POSSIBLE TWO COMPONENT SENSOR KINASE
- TUBERCULIST: Possible two component sensor kinase
- NCBI: Possible two component sensor kinase
- updated information (H37Rv4):
  - gene name: -
  - function:
  - reference:
Type: Not Target
Start: 941190
End: 942467
Operon:

Trans-membrane region:
Role: I.J.2 - Two component systems
GO terms:
- GO:0018106 - peptidyl-histidine phosphorylation (Uniprot)
- GO:0016740 - transferase activity (Uniprot)
- GO:0016310 - phosphorylation (Uniprot)
- GO:0016301 - kinase activity (Uniprot)
- GO:0016021 - integral component of membrane (Uniprot)
- GO:0016020 - membrane (Uniprot)
- GO:0005886 - plasma membrane (Uniprot)
- GO:0005622 - intracellular (Uniprot)
- GO:0004673 - protein histidine kinase activity (Uniprot)
- GO:0000160 - phosphorelay signal transduction system (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020):

PDB aa ident species PDB title

3ZXQ 38% MYCOBACTERIUM TUBERCULOSIS CRYSTAL STRUCTURE OF THE ATP-BINDING DOMAIN OF MYCOBACTERIUM TUBERCULOSIS DOST
Links to additional information on Rv0845:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.983 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

PDB	aa ident	species	PDB title
3ZXQ	38%	MYCOBACTERIUM TUBERCULOSIS	CRYSTAL STRUCTURE OF THE ATP-BINDING DOMAIN OF MYCOBACTERIUM TUBERCULOSIS DOST

Amino Acid Sequence

VPSYGNLGRLGGRHEYGVLVAMTSSAELDRVRWAHQLRSYRIASVLRIGVVGLMVAAMVVGTSRSEWPQQIVLIGVYAVAALWALLLAYSASRRFFALRR
FRSMGRLEPFAFTAVDVLILTGFQLLSTDGIYPLLIMILLPVLVGLDVSTRRAAVVLACTLVGFAVAVLGDPVMLRAIGWPETIFRFALYAFLCATALMV
VRIEERHTRSVAGLSALRAELLAQTMTASEVLQRRIAEAIHDGPLQDVLAARQELIELDAVTPGDERVGRALAGLQSASERLRQATFELHPAVLEQVGLG
PAVKQLAASTAQRSGIKISTDIDYPIRSGIDPIVFGVVRELLSNVVRHSGATTASVRLGITDEKCVLDVADDGVGVTGDTMARRLGEGHIGLASHRARVD
AAGGVLVFLATPRGTHVCVELPLKR

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Uncertain	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.413150; 7 non-insertions in a row out of 27 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 27 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 27 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 5 non-insertions in a row out of 27 sites
Non-Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 5 non-insertions in a row out of 27 sites
No-Data	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Too-Short	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: -1
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv3223c (sigH)
- Rv3286c (sigF)

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv1473A (-) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv3223c (sigH)
- Rv3286c (sigF)

Property	Value	Creator	Evidence	PMID	Comment
Citation	Deletion of two-component regulatory systems increases the virulence of Mycobacterium tuberculosis. T. Parish, DA. Smith et al. Infect. Immun. 2003	hibeeluck	NAS	12595424	Gene neighbourhood
Interaction	Signaling Rv0844c	hibeeluck	NAS		Gene neighbourhood T. Parish, DA. Smith et al. Deletion of two-component regulatory systems increases the virulence of Mycobacterium tuberculosis. Infect. Immun. 2003

TB Genome Annotation Portal