TB Genome Annotation Portal

Rv0758 (phoR)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: two-component system OmpR family sensor kinase
  • REFSEQ: two component system response sensor kinase membrane associated PHOR
  • PATRIC: PhoR
  • TUBERCULIST: Possible two component system response sensor kinase membrane associated PhoR
  • NCBI: Possible two component system response sensor kinase membrane associated PhoR
  • updated information (H37Rv4):
    • gene name: phoR
    • function:
    • reference:
• Type: Not Target
• Start: 852396
• End: 853853
• Operon:


• Trans-membrane region:
  
• Role: I.J.2 - Two component systems
• GO terms:
  • GO:0023014 - signal transduction by protein phosphorylation (Uniprot)
  • GO:0016772 - transferase activity, transferring phosphorus-containing groups (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0016310 - phosphorylation (Uniprot)
  • GO:0016301 - kinase activity (Uniprot)
  • GO:0016021 - integral component of membrane (Uniprot)
  • GO:0016020 - membrane (Uniprot)
  • GO:0007165 - signal transduction (Uniprot)
  • GO:0005622 - intracellular (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0004871 - signal transducer activity (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
  • GO:0000160 - phosphorelay signal transduction system (Uniprot)
  • GO:0000155 - phosphorelay sensor kinase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • AHTD[c] -> 3 PI[c] + DHP[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  5UKY	98%	Mycobacterium tuberculosis	DHp domain of PhoR of M. tuberculosis - native data
  5UKV	98%	Mycobacterium tuberculosis	DHp domain of PhoR of M. tuberculosis - SeMet
  6BLK	40%	Mycobacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)	Mycobacterial sensor histidine kinase MprB
  3SL2	38%	Bacillus subtilis	ATP Forms a Stable Complex with the Essential Histidine Kinase WalK (YycG) Domain
  1BXD	36%	Escherichia coli BL21(DE3)	NMR STRUCTURE OF THE HISTIDINE KINASE DOMAIN OF THE E. COLI OSMOSENSOR ENVZ
  1R62	36%	Escherichia coli	Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)
  4JAU	35%	Thermotoga maritima	Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E)
  4JAS	35%	Thermotoga maritima	Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)

• Links to additional information on phoR:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -0.092 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MARHLRGRLPLRVRLVAATLILVATGLVASGIAVTSMLQHRLTSRIDRVLLEEAQIWAQITLPLAPDPYPGHNPDRPPSRFYVRVISPDGQSYTALNDNT
AIPAVPANNDVGRHPTTLPSIGGSKTLWRAVSVRASDGYLTTVAIDLADVRSTVRSLVLLQVGIGSAVLVVPGVAGYAVVRRSLRPLAEFEQTAAAIGAG
QLDRRVPQWHPRTEVGRLSLALNGMLAQIQRAVASAESSAEKARDSEDRMRQFITDASHELRTPLTTIRGFAELYRQGAARDVGMLLSRIESEASRMGLL
VDDLLLLARLDAHRPLELCRVDLLALASDAAHDARAMDPKRRITLEVLDGPGTPEVLGDESRLRQVLRNLVANAIQHTPESADVTVRVGTEGDDAILEVA
DDGPGMSQEDALRVFERFYRADSSRARASGGTGLGLSIVDSLVAAHGGAVTVTTALGEGCCFRVSLPRVSDVDQLSLTPVVPGPP

Additional Information