Rv0627 (vapC5)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: toxin
- REFSEQ: hypothetical protein
- PATRIC: FIG00821685: hypothetical protein
- TUBERCULIST: Possible toxin VapC5
- NCBI: Possible toxin VapC5
- updated information (H37Rv4):
  - gene name: vapC5
  - function:
  - reference:
Type: Not Target
Start: 718282
End: 718689
Operon:

Trans-membrane region:
Role: V - Conserved hypotheticals
GO terms:
- GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic (Uniprot)
- GO:0090501 - RNA phosphodiester bond hydrolysis (Uniprot)
- GO:0090305 - nucleic acid phosphodiester bond hydrolysis (Uniprot)
- GO:0046872 - metal ion binding (Uniprot)
- GO:0040007 - growth (Uniprot)
- GO:0016787 - hydrolase activity (Uniprot)
- GO:0005576 - extracellular region (Uniprot)
- GO:0004540 - ribonuclease activity (Uniprot)
- GO:0004521 - endoribonuclease activity (Uniprot)
- GO:0004518 - nuclease activity (Uniprot)
- GO:0000287 - magnesium ion binding (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure: 3dbo
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020):

PDB aa ident species PDB title

3DBO 100% Mycobacterium tuberculosis Crystal structure of a member of the VapBC family of toxin-antitoxin systems, VapBC-5, from Mycobacterium tuberculosis
Links to additional information on vapC5:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.175 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

PDB	aa ident	species	PDB title
3DBO	100%	Mycobacterium tuberculosis	Crystal structure of a member of the VapBC family of toxin-antitoxin systems, VapBC-5, from Mycobacterium tuberculosis

Amino Acid Sequence

VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAARMWARLRIHLAESGRRVRIND
LWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Too-Short	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: -1.000000; 1 non-insertions in a row out of 1 sites
Too-Short	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: -1.000000; 1 non-insertions in a row out of 1 sites
Too-Short	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: -1.000000; 1 non-insertions in a row out of 1 sites
Too-Short	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: -1.000000; 1 non-insertions in a row out of 1 sites
Too-Short	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: -1.000000; 1 non-insertions in a row out of 1 sites
Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 0.14
Uncertain	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

RNA processing and modification

Energy production and conversion

Chromatin structure and dynamics

Amino acid transport and metabolism

Cell cycle control, cell division, chromosome partitioning

Carbohydrate transport and metabolism

Nucleotide transport and metabolism

Lipid transport and metabolism

Coenzyme transport and metabolism

Transcription

Translation, ribosomal structure and biogenesis

Cell wall/membrane/envelope biogenesis

Replication, recombination and repair

Posttranslational modification, protein turnover, chaperones

Cell motility

Secondary metabolites biosynthesis, transport and catabolism

Inorganic ion transport and metabolism

Function unknown

General function prediction only

Intracellular trafficking, secretion, and vesicular transport

Signal transduction mechanisms

Extracellular structures

Defense mechanisms

Nuclear structure

Cytoskeleton

BioCyc Co-regulated genes based on gene expression profiling (Systems Biology, Inferelator Network)

Differentially expressed as result of RNASeq in glycerol environment (Only top 20 genes shown sorted by log fold change with p_adj 0.05).

Conditionally essential as result of TNSeq (Only top 20 genes shown sorted by log fold change with p_adj 0.05).

BioCyc Transcription factor binding based on ChIP-Seq (Systems Biology)

Binds To:
- No bindings to other targets were found.
Bound By:
- No bindings from other targets were found.

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- No bindings to other targets were found.

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Not downregulated by other genes.

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv0627 (vapC5)

Property	Value	Creator	Evidence	PMID	Comment
Citation	The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus J. Mol. Biol. 2009	harsharohiratruefriend	IDA	19445953	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Citation	The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus J. Mol. Biol. 2009	harsharohiratruefriend	IEP	19445953	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Citation	Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg J. Biol. Chem. 2009	harsharohiratruefriend	IDA	18952600	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Citation	Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009	harsharohiratruefriend	IDA	19016878	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Citation	Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. authors,HR. Ramage,LE. Connolly,JS. Cox PLoS Genet. 2009	harsharohiratruefriend	IDA	20011113	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Citation	The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus J. Mol. Biol. 2009	harsharohiratruefriend	IDA	19445953	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Citation	Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg J. Biol. Chem. 2009	harsharohiratruefriend	IEP	18952600	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Citation	Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009	harsharohiratruefriend	IEP	19016878	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Citation	Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. authors,HR. Ramage,LE. Connolly,JS. Cox PLoS Genet. 2009	harsharohiratruefriend	IEP	20011113	Operon(Physical Interaction)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Citation	Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. authors,HR. Ramage,LE. Connolly,JS. Cox PLoS Genet. 2009	harsharohiratruefriend	IEP	20011113	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Citation	The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus J. Mol. Biol. 2009	harsharohiratruefriend	IEP	19445953	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Citation	Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg J. Biol. Chem. 2009	harsharohiratruefriend	IDA	18952600	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Citation	Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009	harsharohiratruefriend	IDA	19016878	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Citation	Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. authors,HR. Ramage,LE. Connolly,JS. Cox PLoS Genet. 2009	harsharohiratruefriend	IDA	20011113	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Citation	Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg J. Biol. Chem. 2009	harsharohiratruefriend	IEP	18952600	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Citation	Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009	harsharohiratruefriend	IEP	19016878	Co-expression (Functional linkage)
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Operon(Physical Interaction) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IDA		Co-expression (Functional linkage) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Operon(Physical Interaction) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009
Interaction	InhibitedBy Rv0626	harsharohiratruefriend	IEP		Co-expression (Functional linkage) authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009
Citation	Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. authors,HR. Ramage,LE. Connolly,JS. Cox PLoS Genet. 2009	jlew		20011113	VapC homolog, PIN domain, Not toxic when expressed in Msmeg
Symbol	VapC	jlew			authors,BA. Ahidjo,D. Kuhnert,JL. McKenzie,EE. Machowski,BG. Gordhan,V. Arcus,GL. Abrahams,V. Mizrahi VapC toxins from Mycobacterium tuberculosis are ribonucleases that differentially inhibit growth and are neutralized by cognate VapB antitoxins. PLoS ONE 2011
Citation	VapC toxins from Mycobacterium tuberculosis are ribonucleases that differentially inhibit growth and are neutralized by cognate VapB antitoxins. authors,BA. Ahidjo,D. Kuhnert,JL. McKenzie,EE. Machowski,BG. Gordhan,V. Arcus,GL. Abrahams,V. Mizrahi PLoS ONE 2011	jlew		21738782	None
Symbol	vapC5	jlew			VapBC5 structure. Assay shows the toxin is an Mg-enabled endoribonuclease authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009
Citation	Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg J. Biol. Chem. 2009	jlew		18952600	VapBC5 structure. Assay shows the toxin is an Mg-enabled endoribonuclease
Symbol	VapC5	jlew			We report the heterologous toxicity of these TA loci in Escherichia coli and show that only a few of the M. tuberculosis-encoded toxins can inhibit E. coli growth and have a killing effect. This killing effect can be suppressed by coexpression of the cognate antitoxin. authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Citation	Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009	jlew		19016878	We report the heterologous toxicity of these TA loci in Escherichia coli and show that only a few of the M. tuberculosis-encoded toxins can inhibit E. coli growth and have a killing effect. This killing effect can be suppressed by coexpression of the cognate antitoxin.
Other	start:718282	rslayden			Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv0595c and Rv0665
Other	stop:718689	rslayden			Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv0595c and Rv0665
Other	strand:+	rslayden			Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv0595c and Rv0665

Property

Value

Creator

Evidence

PMID

Comment

Citation

The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus J. Mol. Biol. 2009

harsharohiratruefriend

IDA

19445953

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Operon(Physical Interaction)
authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009

Citation

harsharohiratruefriend

IEP

19445953

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Citation

Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg J. Biol. Chem. 2009

harsharohiratruefriend

IDA

18952600

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Operon(Physical Interaction)
authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009

Citation

Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009

harsharohiratruefriend

IDA

19016878

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Operon(Physical Interaction)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Citation

Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. authors,HR. Ramage,LE. Connolly,JS. Cox PLoS Genet. 2009

harsharohiratruefriend

IDA

20011113

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Operon(Physical Interaction)
authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Co-expression (Functional linkage)
authors,HR. Ramage,LE. Connolly,JS. Cox Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009

Citation

harsharohiratruefriend

IDA

19445953

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Co-expression (Functional linkage)
authors,J. Robson,JL. McKenzie,R. Cursons,GM. Cook,VL. Arcus The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. J. Mol. Biol. 2009

Citation

harsharohiratruefriend

IEP

18952600

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Citation

Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009

harsharohiratruefriend

IEP

19016878

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Operon(Physical Interaction)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Citation

harsharohiratruefriend

IEP

20011113

Operon(Physical Interaction)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Citation

harsharohiratruefriend

IEP

20011113

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Citation

harsharohiratruefriend

IEP

19445953

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Citation

harsharohiratruefriend

IDA

18952600

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Co-expression (Functional linkage)
authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009

Citation

Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009

harsharohiratruefriend

IDA

19016878

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Co-expression (Functional linkage)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Citation

harsharohiratruefriend

IDA

20011113

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Operon(Physical Interaction)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Citation

harsharohiratruefriend

IEP

18952600

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Citation

Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009

harsharohiratruefriend

IEP

19016878

Co-expression (Functional linkage)

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Co-expression (Functional linkage)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Operon(Physical Interaction)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Co-expression (Functional linkage)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IDA

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Co-expression (Functional linkage)
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Interaction

InhibitedBy Rv0626

harsharohiratruefriend

IEP

Citation

jlew

20011113

VapC homolog, PIN domain, Not toxic when expressed in Msmeg

Symbol

VapC

jlew

authors,BA. Ahidjo,D. Kuhnert,JL. McKenzie,EE. Machowski,BG. Gordhan,V. Arcus,GL. Abrahams,V. Mizrahi VapC toxins from Mycobacterium tuberculosis are ribonucleases that differentially inhibit growth and are neutralized by cognate VapB antitoxins. PLoS ONE 2011

Citation

VapC toxins from Mycobacterium tuberculosis are ribonucleases that differentially inhibit growth and are neutralized by cognate VapB antitoxins. authors,BA. Ahidjo,D. Kuhnert,JL. McKenzie,EE. Machowski,BG. Gordhan,V. Arcus,GL. Abrahams,V. Mizrahi PLoS ONE 2011

jlew

21738782

None

Symbol

vapC5

jlew

VapBC5 structure. Assay shows the toxin is an Mg-enabled endoribonuclease
authors,L. Miallau,M. Faller,J. Chiang,M. Arbing,F. Guo,D. Cascio,D. Eisenberg Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J. Biol. Chem. 2009

Citation

jlew

18952600

VapBC5 structure. Assay shows the toxin is an Mg-enabled endoribonuclease

Symbol

VapC5

jlew

We report the heterologous toxicity of these TA loci in Escherichia coli and show that only a few of the M. tuberculosis-encoded toxins can inhibit E. coli growth and have a killing effect. This killing effect can be suppressed by coexpression of the cognate antitoxin.
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Citation

Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009

jlew

19016878

Other

start:718282

rslayden

Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv0595c and Rv0665

Other

stop:718689

rslayden

Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv0595c and Rv0665

Other

strand:+

rslayden

Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv0595c and Rv0665

Comments