Rv0602c (tcrA)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: transcriptional regulatory protein tcrA
- REFSEQ: two component DNA binding transcriptional regulatory protein TCRA
- PATRIC: Two component DNA binding Transcriptional regulatory protein TcrA
- TUBERCULIST: Two component DNA binding transcriptional regulatory protein TcrA
- NCBI: Two component DNA binding transcriptional regulatory protein TcrA
- updated information (H37Rv4):
  - gene name: tcrA
  - function:
  - reference:
Type: Not Target
Start: 699038
End: 699799
Operon:

Trans-membrane region:
Role: I.J.2 - Two component systems
GO terms:
- GO:0070298 - negative regulation of phosphorelay signal transduction system (Uniprot)
- GO:0006355 - regulation of transcription, DNA-templated (Uniprot)
- GO:0006351 - transcription, DNA-templated (Uniprot)
- GO:0005886 - plasma membrane (Uniprot)
- GO:0005737 - cytoplasm (Uniprot)
- GO:0003677 - DNA binding (Uniprot)
- GO:0000160 - phosphorelay signal transduction system (Uniprot)
- GO:0000156 - phosphorelay response regulator activity (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB

Top 10 Homologs in PDB (as of Nov 2020):

PDB	aa ident	species	PDB title
3NNN	50%	Thermotoga maritima	BeF3 Activated DrrD Receiver Domain
4UHS	47%	ESCHERICHIA COLI	Crystal structure of the receiver domain of CpxR from E. coli (tetragonal form)
4UHK	47%	ESCHERICHIA COLI	Crystal structure of the receiver domain of CpxR from E. coli (phosphorylated)
4UHJ	47%	ESCHERICHIA COLI	Crystal structure of the receiver domain of CpxR from E. coli (orthorhombic form)
5UIC	47%	Francisella tularensis subsp. novicida (strain U112)	Structure of the Francisella response regulator receiver domain, QseB
3W9S	46%	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044	Crystal Structure Analysis of the N-terminal Receiver domain of Response Regulator PmrA
1MVO	46%	Bacillus subtilis	Crystal structure of the PhoP receiver domain from Bacillus subtilis
6IS2	45%	Staphylococcus aureus (strain bovine RF122 / ET3-1)	Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in complex with Mg
6IS1	45%	Staphylococcus aureus (strain bovine RF122 / ET3-1)	Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in complex with BeF3 and Mg
6IS3	45%	Staphylococcus aureus RF122	Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain

Links to additional information on tcrA:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.62 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

VADETTMRAGRGPGRACGRVSGVRILVVEDEPKMTALLARALTEEGHTVDTVADGRHAVAAVDGGDYDAVVLDVMLPGIDGFEVCARLRRQRVWTPVLML
TARGAVTDRIAGLDGGADDYLTKPFNLDELFARLRALSRRGPIPRPPTLEAGDLRLDPSEHRVWRADTEIRLSHKEFTLLEALIRRPGIVHTRAQLLERC
WDAAYEARSNIVDVYIRYLRDKIDRPFGVTSLETIRGAGYRLRKDGGRHALPR

(Nucleotide sequence available on KEGG)

Additional Information

Analysis of Positive Selection in Clinical Isolates new

Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
- Moldova: 2,057 clinical isolates
- global set: 5,195 clinical isolates from 15 other countries
In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	YES
omega peak height (95%CI lower bound)	2.34 (0.55)	2.99 (1.42)
codons under selection		51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65
omega plots
genetic variants*	link	link
statistics at each codon	link	link

* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv0602c/tcrA, gene len: 761 bp, num TA sites: 11

condition	dataset	call	medium	method	notes
in-vitro	DeJesus 2017 mBio	non-essential	7H9	HMM	fully saturated, 14 TnSeq libraries combined
in-vitro	Sassetti 2003 Mol Micro	non-essential	7H9	TRASH	essential if hybridization ratio<0.2
in-vivo (mice)	Sassetti 2003 PNAS	no data	BL6 mice	TRASH	essential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)	Griffin 2011 PPath	non-essential	M9 minimal+glycerol	Gumbel	2 replicates; Padj<0.05
in-vitro (cholesterol)	Griffin 2011 PPath	non-essential	M9 minimal+cholesterol	Gumbel	3 replicates; Padj<0.05
differentially essential in cholesterol	Griffin 2011 PPath	NO (LFC=-0.95)	cholesterol vs glycerol	resampling-SR	YES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitro	Smith 2022 eLife	non-essential	7H9	HMM	6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)	Smith 2022 eLife	non-essential	BL6 mice	HMM	6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in mice	Smith 2022 eLife	NO (LFC=-0.206)	in-vivo vs in-vitro	ZINB	YES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)	Minato 2019 mSys	non-essential	minimal medium	HMM
in-vitro (YM rich medium)	Minato 2019 mSys	non-essential	YM rich medium	HMM	7H9 supplemented with ~20 metabolites (amino acids, vitamins)
differentially essential in YM rich medium	Minato 2019 mSys	NO (LFC=0.04)	YM rich vs minimal medium	resampling

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- Rv0157 (pntB) (Direct binding)
- Rv0188 (-) (Direct binding)
- Rv0208c (-) (Direct binding)
- Rv0222 (echA1) (Direct binding)
- Rv0331 (-) (Direct binding)
- Rv0350 (dnaK) (Direct binding)
- Rv0463 (-) (Direct binding)
- Rv0475 (hbhA) (Direct binding)
- Rv0489 (gpm1) (Direct binding)
- Rv0602c (tcrA) (Direct binding)
- Rv0603 (-) (Direct binding)
- Rv0688 (-) (Direct binding)
- Rv0789c (-) (Direct binding)
- Rv1033c (trcR) (Direct binding)
- Rv1116 (-) (Direct binding)
- Rv1170 (mshB) (Direct binding)
- Rv1221 (sigE) (Direct binding)
- Rv1265 (-) (Direct binding)
- Rv1394c (cyp132) (Direct binding)
- Rv1395 (-) (Direct binding)
- Rv1503c (-) (Direct binding)
- Rv1524 (-) (Direct binding)
- Rv1776c (-) (Direct binding)
- Rv1777 (cyp144) (Direct binding)
- Rv1793 (esxN) (Direct binding)
- Rv1799 (lppT) (Direct binding)
- Rv1963c (mce3R) (Direct binding)
- Rv1985c (-) (Direct binding)
- Rv1986 (-) (Direct binding)
- Rv2022c (-) (Direct binding)
- Rv2107 (PE22) (Direct binding)
- Rv2136c (-) (Direct binding)
- Rv2137c (-) (Direct binding)
- Rv2138 (lppL) (Direct binding)
- Rv2145c (wag31) (Direct binding)
- Rv2197c (-) (Direct binding)
- Rv2243 (fabD) (Direct binding)
- Rv2272 (-) (Direct binding)
- Rv2280 (-) (Direct binding)
- Rv2299c (htpG) (Direct binding)
- Rv2307B (-) (Direct binding)
- Rv2333c (stp) (Direct binding)
- Rv2352c (PPE38) (Direct binding)
- Rv2367c (-) (Direct binding)
- Rv2438A (-) (Direct binding)
- Rv2438c (nadE) (Direct binding)
- Rv2452c (-) (Direct binding)
- Rv2562 (-) (Direct binding)
- Rv2571c (-) (Direct binding)
- Rv2588c (yajC) (Direct binding)
- Rv2779c (-) (Direct binding)
- Rv2780 (ald) (Direct binding)
- Rv2787 (-) (Direct binding)
- Rv2839c (infB) (Direct binding)
- Rv2884 (-) (Direct binding)
- Rv2901c (-) (Direct binding)
- Rv2902c (rnhB) (Direct binding)
- Rv2947c (pks15) (Direct binding)
- Rv2999 (lppY) (Direct binding)
- Rv3007c (-) (Direct binding)
- Rv3034c (-) (Direct binding)
- Rv3035 (-) (Direct binding)
- Rv3108 (-) (Direct binding)
- Rv3109 (moaA1) (Direct binding)
- Rv3347c (PPE55) (Direct binding)
- Rv3477 (PE31) (Direct binding)
- Rv3706c (-) (Direct binding)
- Rv3811 (-) (Direct binding)
- Rv3812 (PE_PGRS62) (Direct binding)
- Rv3869 (eccB1) (Direct binding)
- Rv3888c (-) (Direct binding)
- Rv0207c (-) (Upstream of operon)
- Rv0351 (grpE) (Upstream of operon)
- Rv0352 (dnaJ1) (Upstream of operon)
- Rv0353 (hspR) (Upstream of operon)
- Rv1032c (trcS) (Upstream of operon)
- Rv1393c (-) (Upstream of operon)
- Rv2021c (-) (Upstream of operon)
- Rv2135c (-) (Upstream of operon)
- Rv2273 (-) (Upstream of operon)
- Rv2778c (-) (Upstream of operon)
- Rv2836c (dinF) (Upstream of operon)
- Rv2837c (-) (Upstream of operon)
- Rv2838c (rbfA) (Upstream of operon)
- Rv3478 (PPE60) (Upstream of operon)
- Rv3885c (eccE2) (Upstream of operon)
- Rv3886c (mycP2) (Upstream of operon)
- Rv3887c (eccD2) (Upstream of operon)
Bound By:
- Rv0602c (tcrA) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
Upregulated by:
- Rv0602c (tcrA)
- Rv2989 (-)
Downregulates:
Downregulated by:
- Not downregulated by other genes.

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv0602c (tcrA)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Citation	Functional insights from the molecular modelling of a novel two-component system. R. Shrivastava, DR. Das et al. Biochem. Biophys. Res. Commun. 2006	shahanup86	IDA	16650822	Structural Analysis
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Citation	Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. R. Shrivastava, AK. Ghosh et al. Microbiology (Reading, Engl.) 2009	shahanup86	IDA	19246748	Structural Analysis
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Citation	Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. M. Bhattacharya,A. Biswas,AK. Das Biochimie 2010	shahanup86	IDA	19962420	Structural Analysis
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Citation	Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. M. Bhattacharya,A. Biswas,AK. Das Biochimie 2010	shahanup86	IDA	19962420	Spectrophotometric
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Citation	Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. R. Shrivastava, AK. Ghosh et al. FEBS Lett. 2007	shahanup86	IDA	17434492	Structural Analysis
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007
Citation	Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. R. Shrivastava & AK. Das Int. J. Biol. Macromol. 2007	shahanup86	IDA	17335892	Structural Analysis
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Citation	Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. R. Shrivastava & AK. Das Int. J. Biol. Macromol. 2007	shahanup86	IDA	17335892	Spectrophotometric
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Citation	Functional insights from the molecular modelling of a novel two-component system. R. Shrivastava, DR. Das et al. Biochem. Biophys. Res. Commun. 2006	shahanup86	IDA	16650822	Spectrophotometric
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Citation	Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. R. Shrivastava, AK. Ghosh et al. Microbiology (Reading, Engl.) 2009	shahanup86	IDA	19246748	Spectrophotometric
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Citation	Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. R. Shrivastava, AK. Ghosh et al. FEBS Lett. 2007	shahanup86	IDA	17434492	Spectrophotometric
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Interaction	Regulatory Rv0601c	shahanup86	IDA		Structural Analysis R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007
Interaction	Regulatory Rv0601c	shahanup86	IDA		Spectrophotometric R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010
Interaction	Regulatory Rv0600c	shahanup86	IDA		Structural Analysis R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006
Interaction	Regulatory Rv0600c	shahanup86	IDA		Spectrophotometric R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007

Property

Value

Creator

Evidence

PMID

Comment

Interaction

Regulatory Rv0601c

shahanup86

IDA

Structural Analysis
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Interaction

Regulatory Rv0601c

shahanup86

IDA

Structural Analysis
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Citation

Functional insights from the molecular modelling of a novel two-component system. R. Shrivastava, DR. Das et al. Biochem. Biophys. Res. Commun. 2006

shahanup86

IDA

16650822

Structural Analysis

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Interaction

Regulatory Rv0601c

shahanup86

IDA

Structural Analysis
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Citation

Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. R. Shrivastava, AK. Ghosh et al. Microbiology (Reading, Engl.) 2009

shahanup86

IDA

19246748

Structural Analysis

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009

Interaction

Regulatory Rv0601c

shahanup86

IDA

Citation

Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. M. Bhattacharya,A. Biswas,AK. Das Biochimie 2010

shahanup86

IDA

19962420

Structural Analysis

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Interaction

Regulatory Rv0601c

shahanup86

IDA

Spectrophotometric
R. Shrivastava, AK. Ghosh et al. Intra- and intermolecular domain interactions among novel two-component system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2009

Citation

Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. M. Bhattacharya,A. Biswas,AK. Das Biochimie 2010

shahanup86

IDA

19962420

Spectrophotometric

Interaction

Regulatory Rv0600c

shahanup86

IDA

Spectrophotometric
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Interaction

Regulatory Rv0601c

shahanup86

IDA

Spectrophotometric
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Citation

Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. R. Shrivastava, AK. Ghosh et al. FEBS Lett. 2007

shahanup86

IDA

17434492

Structural Analysis

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007

Interaction

Regulatory Rv0601c

shahanup86

IDA

Citation

Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. R. Shrivastava & AK. Das Int. J. Biol. Macromol. 2007

shahanup86

IDA

17335892

Structural Analysis

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Citation

Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. R. Shrivastava & AK. Das Int. J. Biol. Macromol. 2007

shahanup86

IDA

17335892

Spectrophotometric

Interaction

Regulatory Rv0600c

shahanup86

IDA

Spectrophotometric
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Interaction

Regulatory Rv0601c

shahanup86

IDA

Spectrophotometric
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Citation

Functional insights from the molecular modelling of a novel two-component system. R. Shrivastava, DR. Das et al. Biochem. Biophys. Res. Commun. 2006

shahanup86

IDA

16650822

Spectrophotometric

Interaction

Regulatory Rv0600c

shahanup86

IDA

Spectrophotometric
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Interaction

Regulatory Rv0601c

shahanup86

IDA

Spectrophotometric
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Citation

shahanup86

IDA

19246748

Spectrophotometric

Interaction

Regulatory Rv0600c

shahanup86

IDA

Interaction

Regulatory Rv0601c

shahanup86

IDA

Interaction

Regulatory Rv0601c

shahanup86

IDA

Structural Analysis
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Citation

shahanup86

IDA

17434492

Spectrophotometric

Interaction

Regulatory Rv0600c

shahanup86

IDA

Spectrophotometric
R. Shrivastava, AK. Ghosh et al. Probing the nucleotide binding and phosphorylation by the histidine kinase of a novel three-protein two-component system from Mycobacterium tuberculosis. FEBS Lett. 2007

Interaction

Regulatory Rv0601c

shahanup86

IDA

Interaction

Regulatory Rv0601c

shahanup86

IDA

Interaction

Regulatory Rv0601c

shahanup86

IDA

Structural Analysis
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Interaction

Regulatory Rv0601c

shahanup86

IDA

Structural Analysis
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Interaction

Regulatory Rv0601c

shahanup86

IDA

Spectrophotometric
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Interaction

Regulatory Rv0601c

shahanup86

IDA

Interaction

Regulatory Rv0601c

shahanup86

IDA

Spectrophotometric
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Interaction

Regulatory Rv0601c

shahanup86

IDA

Interaction

Regulatory Rv0601c

shahanup86

IDA

Spectrophotometric
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Interaction

Regulatory Rv0600c

shahanup86

IDA

Structural Analysis
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Interaction

Regulatory Rv0600c

shahanup86

IDA

Interaction

Regulatory Rv0600c

shahanup86

IDA

Interaction

Regulatory Rv0600c

shahanup86

IDA

Spectrophotometric
M. Bhattacharya,A. Biswas,AK. Das Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis. Biochimie 2010

Interaction

Regulatory Rv0600c

shahanup86

IDA

Interaction

Regulatory Rv0600c

shahanup86

IDA

Spectrophotometric
R. Shrivastava & AK. Das Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis. Int. J. Biol. Macromol. 2007

Interaction

Regulatory Rv0600c

shahanup86

IDA

Spectrophotometric
R. Shrivastava, DR. Das et al. Functional insights from the molecular modelling of a novel two-component system. Biochem. Biophys. Res. Commun. 2006

Interaction

Regulatory Rv0600c

shahanup86

IDA

Comments

TB Genome Annotation Portal

Rv0602c (tcrA)

Amino Acid Sequence

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

Interactions based on ChIPSeq data

Interactions based on ChIPSeq data (Minch et al. 2014)

Interactions based on TFOE data (Rustad et al. 2014)

TBCAP

Comments

Analysis of Positive Selection in Clinical Isolates new