TB Genome Annotation Portal

Rv0440 (groEL2)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: chaperonin 1
  • REFSEQ: chaperonin GroEL
  • PATRIC: Heat shock protein 60 family chaperone GroEL
  • TUBERCULIST: 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A)
  • NCBI: 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A)
  • updated information (H37Rv4):
    • gene name: groEL2
    • function:
    • reference:
• Type: Not Target
• Start: 528608
• End: 530230
• Operon:


• Trans-membrane region:
  
• Role: III.B - Chaperones/Heat shock
• GO terms:
  • GO:2000679 - positive regulation of transcription regulatory region DNA binding (Uniprot)
  • GO:1990220 - GroEL-GroES complex (Uniprot)
  • GO:0061077 - chaperone-mediated protein folding (Uniprot)
  • GO:0051082 - unfolded protein binding (Uniprot)
  • GO:0044406 - adhesion of symbiont to host (Uniprot)
  • GO:0044183 - protein binding involved in protein folding (Uniprot)
  • GO:0042603 - capsule (Uniprot)
  • GO:0042026 - protein refolding (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0009408 - response to heat (Uniprot)
  • GO:0006458 - 'de novo' protein folding (Uniprot)
  • GO:0006457 - protein folding (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0005576 - extracellular region (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0001666 - response to hypoxia (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 1sjp, 3rtk
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  3RTK	100%	Mycobacterium tuberculosis	Crystal structure of Cpn60.2 from Mycobacterium tuberculosis at 2.8A
  1SJP	100%	Mycobacterium tuberculosis	Mycobacterium tuberculosis Chaperonin60.2
  1SRV	70%	Thermus thermophilus	THERMUS THERMOPHILUS GROEL (HSP60 CLASS) FRAGMENT (APICAL DOMAIN) COMPRISING RESIDUES 192-336
  1FY9	67%	Escherichia coli	CRYSTAL STRUCTURE OF THE HEXA-SUBSTITUTED MUTANT OF THE MOLECULAR CHAPERONIN GROEL APICAL DOMAIN
  1FYA	66%	Escherichia coli	CRYSTAL STRUCTURE OF THE HEXA-SUBSTITUTED MUTANT OF THE MOLECULAR CHAPERONIN GROEL APICAL DOMAIN
  3VZ8	66%	Escherichia coli	Crystal Structure Analysis of the Mini-chaperonin variant with Leu 185, Val 186, Pro 187, Arg 188 and Ser 190 replaced with all Gly
  3VZ6	66%	Escherichia coli	Crystal Structure Analysis of the Mini-chaperonines, variant with Gly 184 replaced with Ile and Leu 185 replaced Val and Val 186 replaced with Leu.
  3VZ7	66%	Escherichia coli	Crystal Structure Analysis of the mini-chaperonin variant with Pro 187 Gly
  3OSX	66%	Xenorhabdus nematophila	Crystal Structure of Apical Domain of Insecticidal GroEL from Xenorhapdus nematophila
  1LA1	66%	Escherichia coli	Gro-EL Fragment (Apical Domain) Comprising Residues 188-379

• Links to additional information on groEL2:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -11.617 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MAKTIAYDEEARRGLERGLNALADAVKVTLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVR
EGLRNVAAGANPLGLKRGIEKAVEKVTETLLKGAKEVETKEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISG
YFVTDPERQEAVLEDPYILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIAEDVEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAILTGGQVIS
EEVGLTLENADLSLLGKARKVVVTKDETTIVEGAGDTDAIAGRVAQIRQEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRN
AKAAVEEGIVAGGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPLKQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLAAGVADPVKVTRSA
LQNAASIAGLFLTTEAVVADKPEKEKASVPGGGDMGGMDF

Additional Information