Rv0014c (pknB)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: serine/threonine-protein kinase pknB
- REFSEQ: transmembrane serine/threonine-protein kinase B PKNB (protein kinase B) (STPK B)
- PATRIC: Serine/threonine-protein kinase PknB (EC 2.7.11.1)
- TUBERCULIST: Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B)
- NCBI: Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B)
- updated information (H37Rv4):
  - gene name: pknB
  - function:
  - reference:
Type: Not Target
Start: 15590
End: 17470
Operon:

Trans-membrane region:
Role: I.J.3 - Serine-Threonine protein kinases and phosphoprotein phosphatases
GO terms:
- GO:0052572 - response to host immune response (Uniprot)
- GO:0046872 - metal ion binding (Uniprot)
- GO:0046777 - protein autophosphorylation (Uniprot)
- GO:0045717 - negative regulation of fatty acid biosynthetic process (Uniprot)
- GO:0043388 - positive regulation of DNA binding (Uniprot)
- GO:0043086 - negative regulation of catalytic activity (Uniprot)
- GO:0043085 - positive regulation of catalytic activity (Uniprot)
- GO:0042802 - identical protein binding (Uniprot)
- GO:0040007 - growth (Uniprot)
- GO:0032091 - negative regulation of protein binding (Uniprot)
- GO:0030145 - manganese ion binding (Uniprot)
- GO:0016740 - transferase activity (Uniprot)
- GO:0016310 - phosphorylation (Uniprot)
- GO:0016301 - kinase activity (Uniprot)
- GO:0016021 - integral component of membrane (Uniprot)
- GO:0016020 - membrane (Uniprot)
- GO:0010698 - acetyltransferase activator activity (Uniprot)
- GO:0009405 - pathogenesis (Uniprot)
- GO:0009252 - peptidoglycan biosynthetic process (Uniprot)
- GO:0008360 - regulation of cell shape (Uniprot)
- GO:0006468 - protein phosphorylation (Uniprot)
- GO:0005886 - plasma membrane (Uniprot)
- GO:0005618 - cell wall (Uniprot)
- GO:0005524 - ATP binding (Uniprot)
- GO:0005515 - protein binding (Uniprot)
- GO:0004674 - protein serine/threonine kinase activity (Uniprot)
- GO:0004672 - protein kinase activity (Uniprot)
- GO:0000166 - nucleotide binding (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure: 3ork, 3ori, 3oro, 3orm, 3orl, 3ouv, 3orp, 3ort, 3f69, 1o6y, 3f61, 2kui, 1mru, 2kue, 2kud, 2fum, 2kuf
Search for Homologs in PDB

Top 10 Homologs in PDB (as of Nov 2020):

PDB	aa ident	species	PDB title
6B2P	100%	Mycobacterium tuberculosis	Dual Inhibition of the Essential Protein Kinases A and B in Mycobacterium tuberculosis
5U94	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal structure of the Mycobacterium tuberculosis PASTA kinase PknB in complex with the potential theraputic kinase inhibitor GSK690693.
5E12	100%	Mycobacterium tuberculosis	Crystal Structure of PASTA Domains 2, 3 and 4 of Mycobacterium tuberculosis Protein Kinase B
5E10	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal Structure of PASTA Domains 1 and 2 of Mycobacterium tuberculosis Protein Kinase B
5E0Z	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal Structure of PASTA Domains 3 and 4 of Mycobacterium tuberculosis Protein Kinase B
5E0Y	100%	Mycobacterium tuberculosis	Crystal Structure of PASTA Domain 4 of Mycobacterium tuberculosis Protein Kinase B
2KUI	100%	Mycobacterium tuberculosis	NMR structure of the PASTA domain of Mycobacterium tuberculosis of PknB
2KUF	100%	Mycobacterium tuberculosis	NMR structure of the PASTA domain 3 and 4 of Mycobacterium tuberculosis of PknB
2KUE	100%	Mycobacterium tuberculosis	NMR structure of the PASTA domain 2 and 3 of Mycobacterium tuberculosis of PknB
2KUD	100%	Mycobacterium tuberculosis	NMR structure of the PASTA domain 1 and 2 of Mycobacterium tuberculosis of PknB

Links to additional information on pknB:
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = -8.232 (from Jeremy Rock's lab)

Amino Acid Sequence

MTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETPAGPLPYIVMEYVDGVTL
RDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIHRDVKPANIMISATNAVKVMDFGIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVY
SLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPSARHEGLSADLDAVVLKALAKNPENRYQTAAEMRADLVRVHNGEPPEAPKVLTDAERTSLLSSA
AGNLSGPRTDPLPRQDLDDTDRDRSIGSVGRWVAVVAVLAVLTVVVTIAINTFGGITRDVQVPDVRGQSSADAIATLQNRGFKIRTLQKPDSTIPPDHVI
GTDPAANTSVSAGDEITVNVSTGPEQREIPDVSTLTYAEAVKKLTAAGFGRFKQANSPSTPELVGKVIGTNPPANQTSAITNVVIIIVGSGPATKDIPDV
AGQTVDVAQKNLNVYGFTKFSQASVDSPRPAGEVTGTNPPAGTTVPVDSVIELQVSKGNQFVMPDLSGMFWVDAEPRLRALGWTGMLDKGADVDAGGSQH
NRVVYQNPPAGTGVNRDGIITLRFGQ

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 1.000000; 24 non-insertions in a row out of 24 sites
Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 1.000000; 24 non-insertions in a row out of 24 sites
Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 1.000000; 24 non-insertions in a row out of 24 sites
Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 1.000000; 24 non-insertions in a row out of 24 sites
Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 1.000000; 24 non-insertions in a row out of 24 sites
Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 0.05
Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

RNA processing and modification

Energy production and conversion

Chromatin structure and dynamics

Amino acid transport and metabolism

Cell cycle control, cell division, chromosome partitioning

Carbohydrate transport and metabolism

Nucleotide transport and metabolism

Lipid transport and metabolism

Coenzyme transport and metabolism

Transcription

Translation, ribosomal structure and biogenesis

Cell wall/membrane/envelope biogenesis

Replication, recombination and repair

Posttranslational modification, protein turnover, chaperones

Cell motility

Secondary metabolites biosynthesis, transport and catabolism

Inorganic ion transport and metabolism

Function unknown

General function prediction only

Intracellular trafficking, secretion, and vesicular transport

Signal transduction mechanisms

Extracellular structures

Defense mechanisms

Nuclear structure

Cytoskeleton

BioCyc Co-regulated genes based on gene expression profiling (Systems Biology, Inferelator Network)

Differentially expressed as result of RNASeq in glycerol environment (Only top 20 genes shown sorted by log fold change with p_adj 0.05).

Conditionally essential as result of TNSeq (Only top 20 genes shown sorted by log fold change with p_adj 0.05).

BioCyc Transcription factor binding based on ChIP-Seq (Systems Biology)

Binds To:
- No bindings to other targets were found.
Bound By:
- No bindings from other targets were found.

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0678 (-) (Direct binding)
- Rv1776c (-) (Direct binding)
- Rv2011c (-) (Direct binding)
- Rv0135c (-) (Upstream of operon)
- Rv1353c (-) (Upstream of operon)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Not downregulated by other genes.

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv0014c (pknB)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	Regulatory Rv3223c	sourish10	TAS		Co-expression (Functional linkage) T. Song, SL. Dove et al. RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH. Mol. Microbiol. 2003
Interaction	Regulatory Rv3223c	sourish10	IEP		Co-expression (Functional linkage) P. Sachdeva,R. Misra,AK. Tyagi,Y. Singh The sigma factors of Mycobacterium tuberculosis: regulation of the regulators. FEBS J. 2010
Interaction	Regulatory Rv3223c	sourish10	IEP		Co-expression (Functional linkage) T. Song, SL. Dove et al. RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH. Mol. Microbiol. 2003
Interaction	Regulatory Rv3223c	sourish10	TAS		Co-expression (Functional linkage) P. Sachdeva,R. Misra,AK. Tyagi,Y. Singh The sigma factors of Mycobacterium tuberculosis: regulation of the regulators. FEBS J. 2010
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) authors,OW. Schonecke,W. Schffel Evaluation of combined pharmacological and psychotherapeutic treatment in patients with functional abdominal disorders. Psychother Psychosom 1975
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) authors,PW. Hermans,F. Abebe,VI. Kuteyi,AH. Kolk,JE. Thole,M. Harboe Molecular and immunological characterization of the highly conserved antigen 84 from Mycobacterium tuberculosis and Mycobacterium leprae. Infect. Immun. 1995
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) authors,D. Bucens,MC. Pain Influence of hematocrit, blood gas tensions, and pH on pressure-flow relations in the isolated canine lung. Circ. Res. 1975
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) authors,RJ. Smith,RG. Bryant Metal substitutions incarbonic anhydrase: a halide ion probe study. Biochem. Biophys. Res. Commun. 1975
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) authors,H. Autrup,GP. Warwick Some characteristics of two azoreductase systems in rat liver. Relevance to the activity of 2-[4'-di(2-bromopropyl)-aminophenylazo]benzoic acid (CB10-252), a compound possessing latent cytotoxic activity. Chem. Biol. Interact. 1975
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) authors,JM. Stein The effect of adrenaline and of alpha- and beta-adrenergic blocking agents on ATP concentration and on incorporation of 32Pi into ATP in rat fat cells. Biochem. Pharmacol. 1975
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) authors,K. Hamasha,MB. Sahana,C. Jani,S. Nyayapathy,CM. Kang,SJ. Rehse The effect of Wag31 phosphorylation on the cells and the cell envelope fraction of wild-type and conditional mutants of Mycobacterium smegmatis studied by visible-wavelength Raman spectroscopy. Biochem. Biophys. Res. Commun. 2010
Interaction	Signaling Rv2145c	harsharohiratruefriend	IMP		Affinity purification (Physical interaction) CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Interaction	ActivatedBy Rv1827	madhulsbu	IDA		Spectrophotometric A. Villarino, R. Duran et al. Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. J. Mol. Biol. 2005
Interaction	ActivatedBy Rv1827	madhulsbu	IDA		Structural Analysis A. Villarino, R. Duran et al. Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. J. Mol. Biol. 2005
Interaction	Signaling Rv1436	yashabhasin	IDA		Spectrophotometric authors,H. Nrnberger [Quantitative colorimetric determination of 1-(4-cyclohexylureidophenoxy)-2-hydroxy-3-tert butylaminopropane (talinolol Cordanum, 02-115) in body fluids]. Pharmazie 1975
Interaction	Signaling Rv1436	yashabhasin	IDA		Spectrophotometric authors,BK. Ratha,MS. Kanungo Induction of particulate and soluble isoenzymes of tyrosine aminotransferase by hydrocortisone in the liver of rats as a function of age. Biochem. Biophys. Res. Commun. 1977
Interaction	Signaling Rv1436	yashabhasin	IDA		Structural Analysis authors,KV. Kaloustian,JJ. Poluhowich The role of organic phosphates in modulating the oxygenation behavior of eel hemoglobin. Comp Biochem Physiol A Comp Physiol 1976
Interaction	Signaling Rv1436	yashabhasin	IDA		Structural Analysis authors,H. Nrnberger [Quantitative colorimetric determination of 1-(4-cyclohexylureidophenoxy)-2-hydroxy-3-tert butylaminopropane (talinolol Cordanum, 02-115) in body fluids]. Pharmazie 1975
Interaction	Signaling Rv1436	yashabhasin	IDA		Structural Analysis authors,BK. Ratha,MS. Kanungo Induction of particulate and soluble isoenzymes of tyrosine aminotransferase by hydrocortisone in the liver of rats as a function of age. Biochem. Biophys. Res. Commun. 1977
Interaction	Signaling Rv1436	yashabhasin	IDA		Spectrophotometric authors,KV. Kaloustian,JJ. Poluhowich The role of organic phosphates in modulating the oxygenation behavior of eel hemoglobin. Comp Biochem Physiol A Comp Physiol 1976
Interaction	Inhibits Rv1222	harsharohiratruefriend	IDA		mass spectrometry S. Barik, K. Sureka et al. RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis, is inactivated by phosphorylation-dependent ClpC1P2 proteolysis. Mol. Microbiol. 2010
Interaction	Inhibits Rv1222	harsharohiratruefriend	IDA		mass spectrometry R. Manganelli,R. Provvedi,S. Rodrigue,J. Beaucher,L. Gaudreau,I. Smith,R. Proveddi Sigma factors and global gene regulation in Mycobacterium tuberculosis. J. Bacteriol. 2004
Interaction	PhysicalInteraction Rv1018c	ashwinigbhat	IDA		Structural Analysis W. Zhang, VC. Jones et al. Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase. Int. J. Biochem. Cell Biol. 2008
Interaction	PhysicalInteraction Rv1018c	ashwinigbhat	IDA		Structural Analysis A. Parikh, SK. Verma et al. PknB-Mediated Phosphorylation of a Novel Substrate, N-Acetylglucosamine-1-Phosphate Uridyltransferase, Modulates Its Acetyltransferase Activity. J. Mol. Biol. 2008
Interaction	PhysicalInteraction Rv1018c	priti.priety	IDA		Structural Analysis W. Zhang, VC. Jones et al. Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase. Int. J. Biochem. Cell Biol. 2008
Interaction	PhysicalInteraction Rv1018c	priti.priety	IDA		Structural Analysis A. Parikh, SK. Verma et al. PknB-Mediated Phosphorylation of a Novel Substrate, N-Acetylglucosamine-1-Phosphate Uridyltransferase, Modulates Its Acetyltransferase Activity. J. Mol. Biol. 2008
Interaction	Signaling Rv0019c	vmevada102	IDA		Spectrophotometric Analysis M. Gupta,A. Sajid,G. Arora,V. Tandon,Y. Singh Forkhead-associated domain-containing protein Rv0019c and polyketide-associated protein PapA5, from substrates of serine/threonine protein kinase PknB to interacting proteins of Mycobacterium tuberculosis. J. Biol. Chem. 2009
Interaction	Signaling Rv0020c	vmevada102	IEP		Affinity purification (Physical interaction) C. Grundner, LM. Gay et al. Mycobacterium tuberculosis serine/threonine kinases PknB, PknD, PknE, and PknF phosphorylate multiple FHA domains. Protein Sci. 2005
Interaction	Signaling Rv0018c	vmevada102	IDA		Spectrophotometric Analysis P. Chopra, B. Singh et al. Phosphoprotein phosphatase of Mycobacterium tuberculosis dephosphorylates serine-threonine kinases PknA and PknB. Biochem. Biophys. Res. Commun. 2003
Interaction	Signaling Rv0018c	vmevada102	IDA		Spectrophotometric Analysis B. Boitel, M. Ortiz-Lombarda et al. PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis. Mol. Microbiol. 2003
Interaction	Signaling Rv0015c	vmevada102	IEP		Functional linkage (Co-expression) CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Interaction	Activation Rv0016c	vmevada102	IEP		Co-expression (Functional linkage) A. Dasgupta, P. Datta et al. The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division. Microbiology (Reading, Engl.) 2006
Citation	Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. A. Villarino, R. Duran et al. J. Mol. Biol. 2005	akankshajain.21	IDA	15978616	Spectrophotometric
Interaction	Signaling Rv1827	akankshajain.21	IDA		Spectrophotometric A. Villarino, R. Duran et al. Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. J. Mol. Biol. 2005
Citation	Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. A. Villarino, R. Duran et al. J. Mol. Biol. 2005	akankshajain.21	IDA	15978616	Structural Analysis
Interaction	Signaling Rv1827	akankshajain.21	IDA		Structural Analysis A. Villarino, R. Duran et al. Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. J. Mol. Biol. 2005
Interaction	Signaling Rv0015c	vmevada102	IEP		Functional linkage (Co-expression) A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Citation	Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007	vmevada102	IDA	17148687	Structural Analysis
Interaction	Signaling Rv0015c	vmevada102	IDA		Structural Analysis A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Citation	The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005	vmevada102	IDA	15985609	Structural Analysis
Interaction	Signaling Rv0015c	vmevada102	IDA		Structural Analysis CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Citation	The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005	vmevada102	IPI	15985609	Spectrophotometric
Interaction	Signaling Rv0015c	vmevada102	IPI		Spectrophotometric CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Citation	Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007	vmevada102	IDA	17148687	Spectrophotometric
Interaction	Signaling Rv0015c	vmevada102	IDA		Spectrophotometric A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Citation	The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005	vmevada102	IDA	15985609	Spectrophotometric
Interaction	Signaling Rv0015c	vmevada102	IDA		Spectrophotometric CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Citation	Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007	vmevada102	IPI	17148687	Structural Analysis
Interaction	Signaling Rv0015c	vmevada102	IPI		Structural Analysis A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Citation	The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005	vmevada102	IPI	15985609	Structural Analysis
Interaction	Signaling Rv0015c	vmevada102	IPI		Structural Analysis CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Citation	Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007	vmevada102	IPI	17148687	Affinity purification (Physical interaction)
Interaction	Signaling Rv0015c	vmevada102	IPI		Affinity purification (Physical interaction) A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Citation	The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005	vmevada102	IPI	15985609	Affinity purification (Physical interaction)
Interaction	Signaling Rv0015c	vmevada102	IPI		Affinity purification (Physical interaction) CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Citation	Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007	vmevada102	IDA	17148687	Affinity purification (Physical interaction)
Interaction	Signaling Rv0015c	vmevada102	IDA		Affinity purification (Physical interaction) A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Citation	The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005	vmevada102	IDA	15985609	Affinity purification (Physical interaction)
Interaction	Signaling Rv0015c	vmevada102	IDA		Affinity purification (Physical interaction) CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005
Citation	Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007	vmevada102	IPI	17148687	Spectrophotometric
Interaction	Signaling Rv0015c	vmevada102	IPI		Spectrophotometric A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Citation	The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division. A. Dasgupta, P. Datta et al. Microbiology (Reading, Engl.) 2006	vmevada102	IEP	16436437	Co-expression (Functional linkage)
Interaction	Signaling Rv0016c	vmevada102	IEP		Co-expression (Functional linkage) A. Dasgupta, P. Datta et al. The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division. Microbiology (Reading, Engl.) 2006
Citation	Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007	vmevada102	IEP	17148687	Co-expression (Functional linkage)
Interaction	Signaling Rv0016c	vmevada102	IEP		Co-expression (Functional linkage) A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007
Name	TRANSMEMBRANE SERINE/THREONINE-PROTEIN KINASE, known to phosphorylate InhA;KasA, KasB	mjackson	IDA		Regulatory proteins

Property

Value

Creator

Evidence

PMID

Comment

Interaction

Regulatory Rv3223c

sourish10

TAS

Co-expression (Functional linkage)
T. Song, SL. Dove et al. RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH. Mol. Microbiol. 2003

Interaction

Regulatory Rv3223c

sourish10

IEP

Co-expression (Functional linkage)
P. Sachdeva,R. Misra,AK. Tyagi,Y. Singh The sigma factors of Mycobacterium tuberculosis: regulation of the regulators. FEBS J. 2010

Interaction

Regulatory Rv3223c

sourish10

IEP

Co-expression (Functional linkage)
T. Song, SL. Dove et al. RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH. Mol. Microbiol. 2003

Interaction

Regulatory Rv3223c

sourish10

TAS

Co-expression (Functional linkage)
P. Sachdeva,R. Misra,AK. Tyagi,Y. Singh The sigma factors of Mycobacterium tuberculosis: regulation of the regulators. FEBS J. 2010

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
authors,OW. Schonecke,W. Schffel Evaluation of combined pharmacological and psychotherapeutic treatment in patients with functional abdominal disorders. Psychother Psychosom 1975

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
authors,PW. Hermans,F. Abebe,VI. Kuteyi,AH. Kolk,JE. Thole,M. Harboe Molecular and immunological characterization of the highly conserved antigen 84 from Mycobacterium tuberculosis and Mycobacterium leprae. Infect. Immun. 1995

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
authors,D. Bucens,MC. Pain Influence of hematocrit, blood gas tensions, and pH on pressure-flow relations in the isolated canine lung. Circ. Res. 1975

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
authors,RJ. Smith,RG. Bryant Metal substitutions incarbonic anhydrase: a halide ion probe study. Biochem. Biophys. Res. Commun. 1975

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
authors,H. Autrup,GP. Warwick Some characteristics of two azoreductase systems in rat liver. Relevance to the activity of 2-[4'-di(2-bromopropyl)-aminophenylazo]benzoic acid (CB10-252), a compound possessing latent cytotoxic activity. Chem. Biol. Interact. 1975

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
authors,JM. Stein The effect of adrenaline and of alpha- and beta-adrenergic blocking agents on ATP concentration and on incorporation of 32Pi into ATP in rat fat cells. Biochem. Pharmacol. 1975

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
authors,K. Hamasha,MB. Sahana,C. Jani,S. Nyayapathy,CM. Kang,SJ. Rehse The effect of Wag31 phosphorylation on the cells and the cell envelope fraction of wild-type and conditional mutants of Mycobacterium smegmatis studied by visible-wavelength Raman spectroscopy. Biochem. Biophys. Res. Commun. 2010

Interaction

Signaling Rv2145c

harsharohiratruefriend

IMP

Affinity purification (Physical interaction)
CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005

Interaction

ActivatedBy Rv1827

madhulsbu

IDA

Spectrophotometric
A. Villarino, R. Duran et al. Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. J. Mol. Biol. 2005

Interaction

ActivatedBy Rv1827

madhulsbu

IDA

Structural Analysis
A. Villarino, R. Duran et al. Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. J. Mol. Biol. 2005

Interaction

Signaling Rv1436

yashabhasin

IDA

Spectrophotometric
authors,H. Nrnberger [Quantitative colorimetric determination of 1-(4-cyclohexylureidophenoxy)-2-hydroxy-3-tert butylaminopropane (talinolol Cordanum, 02-115) in body fluids]. Pharmazie 1975

Interaction

Signaling Rv1436

yashabhasin

IDA

Spectrophotometric
authors,BK. Ratha,MS. Kanungo Induction of particulate and soluble isoenzymes of tyrosine aminotransferase by hydrocortisone in the liver of rats as a function of age. Biochem. Biophys. Res. Commun. 1977

Interaction

Signaling Rv1436

yashabhasin

IDA

Structural Analysis
authors,KV. Kaloustian,JJ. Poluhowich The role of organic phosphates in modulating the oxygenation behavior of eel hemoglobin. Comp Biochem Physiol A Comp Physiol 1976

Interaction

Signaling Rv1436

yashabhasin

IDA

Structural Analysis
authors,H. Nrnberger [Quantitative colorimetric determination of 1-(4-cyclohexylureidophenoxy)-2-hydroxy-3-tert butylaminopropane (talinolol Cordanum, 02-115) in body fluids]. Pharmazie 1975

Interaction

Signaling Rv1436

yashabhasin

IDA

Structural Analysis
authors,BK. Ratha,MS. Kanungo Induction of particulate and soluble isoenzymes of tyrosine aminotransferase by hydrocortisone in the liver of rats as a function of age. Biochem. Biophys. Res. Commun. 1977

Interaction

Signaling Rv1436

yashabhasin

IDA

Spectrophotometric
authors,KV. Kaloustian,JJ. Poluhowich The role of organic phosphates in modulating the oxygenation behavior of eel hemoglobin. Comp Biochem Physiol A Comp Physiol 1976

Interaction

Inhibits Rv1222

harsharohiratruefriend

IDA

mass spectrometry
S. Barik, K. Sureka et al. RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis, is inactivated by phosphorylation-dependent ClpC1P2 proteolysis. Mol. Microbiol. 2010

Interaction

Inhibits Rv1222

harsharohiratruefriend

IDA

mass spectrometry
R. Manganelli,R. Provvedi,S. Rodrigue,J. Beaucher,L. Gaudreau,I. Smith,R. Proveddi Sigma factors and global gene regulation in Mycobacterium tuberculosis. J. Bacteriol. 2004

Interaction

PhysicalInteraction Rv1018c

ashwinigbhat

IDA

Structural Analysis
W. Zhang, VC. Jones et al. Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase. Int. J. Biochem. Cell Biol. 2008

Interaction

PhysicalInteraction Rv1018c

ashwinigbhat

IDA

Structural Analysis
A. Parikh, SK. Verma et al. PknB-Mediated Phosphorylation of a Novel Substrate, N-Acetylglucosamine-1-Phosphate Uridyltransferase, Modulates Its Acetyltransferase Activity. J. Mol. Biol. 2008

Interaction

PhysicalInteraction Rv1018c

priti.priety

IDA

Interaction

PhysicalInteraction Rv1018c

priti.priety

IDA

Interaction

Signaling Rv0019c

vmevada102

IDA

Spectrophotometric Analysis
M. Gupta,A. Sajid,G. Arora,V. Tandon,Y. Singh Forkhead-associated domain-containing protein Rv0019c and polyketide-associated protein PapA5, from substrates of serine/threonine protein kinase PknB to interacting proteins of Mycobacterium tuberculosis. J. Biol. Chem. 2009

Interaction

Signaling Rv0020c

vmevada102

IEP

Affinity purification (Physical interaction)
C. Grundner, LM. Gay et al. Mycobacterium tuberculosis serine/threonine kinases PknB, PknD, PknE, and PknF phosphorylate multiple FHA domains. Protein Sci. 2005

Interaction

Signaling Rv0018c

vmevada102

IDA

Spectrophotometric Analysis
P. Chopra, B. Singh et al. Phosphoprotein phosphatase of Mycobacterium tuberculosis dephosphorylates serine-threonine kinases PknA and PknB. Biochem. Biophys. Res. Commun. 2003

Interaction

Signaling Rv0018c

vmevada102

IDA

Spectrophotometric Analysis
B. Boitel, M. Ortiz-Lombarda et al. PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis. Mol. Microbiol. 2003

Interaction

Signaling Rv0015c

vmevada102

IEP

Functional linkage (Co-expression)
CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005

Interaction

Activation Rv0016c

vmevada102

IEP

Co-expression (Functional linkage)
A. Dasgupta, P. Datta et al. The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division. Microbiology (Reading, Engl.) 2006

Citation

Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions. A. Villarino, R. Duran et al. J. Mol. Biol. 2005

akankshajain.21

IDA

15978616

Spectrophotometric

Interaction

Signaling Rv1827

akankshajain.21

IDA

Citation

akankshajain.21

IDA

15978616

Structural Analysis

Interaction

Signaling Rv1827

akankshajain.21

IDA

Interaction

Signaling Rv0015c

vmevada102

IEP

Functional linkage (Co-expression)
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Citation

Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007

vmevada102

IDA

17148687

Structural Analysis

Interaction

Signaling Rv0015c

vmevada102

IDA

Structural Analysis
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Citation

The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005

vmevada102

IDA

15985609

Structural Analysis

Interaction

Signaling Rv0015c

vmevada102

IDA

Structural Analysis
CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005

Citation

The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005

vmevada102

IPI

15985609

Spectrophotometric

Interaction

Signaling Rv0015c

vmevada102

IPI

Spectrophotometric
CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005

Citation

Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007

vmevada102

IDA

17148687

Spectrophotometric

Interaction

Signaling Rv0015c

vmevada102

IDA

Spectrophotometric
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Citation

The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005

vmevada102

IDA

15985609

Spectrophotometric

Interaction

Signaling Rv0015c

vmevada102

IDA

Spectrophotometric
CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005

Citation

Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007

vmevada102

IPI

17148687

Structural Analysis

Interaction

Signaling Rv0015c

vmevada102

IPI

Structural Analysis
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Citation

The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005

vmevada102

IPI

15985609

Structural Analysis

Interaction

Signaling Rv0015c

vmevada102

IPI

Structural Analysis
CM. Kang, DW. Abbott et al. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005

Citation

Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007

vmevada102

IPI

17148687

Affinity purification (Physical interaction)

Interaction

Signaling Rv0015c

vmevada102

IPI

Affinity purification (Physical interaction)
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Citation

The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005

vmevada102

IPI

15985609

Affinity purification (Physical interaction)

Interaction

Signaling Rv0015c

vmevada102

IPI

Citation

Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007

vmevada102

IDA

17148687

Affinity purification (Physical interaction)

Interaction

Signaling Rv0015c

vmevada102

IDA

Affinity purification (Physical interaction)
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Citation

The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. CM. Kang, DW. Abbott et al. Genes Dev. 2005

vmevada102

IDA

15985609

Affinity purification (Physical interaction)

Interaction

Signaling Rv0015c

vmevada102

IDA

Citation

Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007

vmevada102

IPI

17148687

Spectrophotometric

Interaction

Signaling Rv0015c

vmevada102

IPI

Spectrophotometric
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Citation

The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division. A. Dasgupta, P. Datta et al. Microbiology (Reading, Engl.) 2006

vmevada102

IEP

16436437

Co-expression (Functional linkage)

Interaction

Signaling Rv0016c

vmevada102

IEP

Citation

Serine threonine protein kinases of mycobacterial genus: phylogeny to function. A. Narayan, P. Sachdeva et al. Physiol. Genomics 2007

vmevada102

IEP

17148687

Co-expression (Functional linkage)

Interaction

Signaling Rv0016c

vmevada102

IEP

Co-expression (Functional linkage)
A. Narayan, P. Sachdeva et al. Serine threonine protein kinases of mycobacterial genus: phylogeny to function. Physiol. Genomics 2007

Name

TRANSMEMBRANE SERINE/THREONINE-PROTEIN KINASE, known to phosphorylate InhA;KasA, KasB

mjackson

IDA

Regulatory proteins

Comments