TB Genome Annotation Portal

Rv0013 (trpG)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: anthranilate synthase component 2
  • REFSEQ: para-aminobenzoate synthase component II
  • PATRIC: Anthranilate synthase amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase amidotransferase component (EC 6.3.5.8)
  • TUBERCULIST: Possible anthranilate synthase component II TrpG (glutamine amidotransferase)
  • NCBI: Possible anthranilate synthase component II TrpG (glutamine amidotransferase)
  • updated information (H37Rv4):
    • gene name: trpG
    • function: "trpG is a bifunctional enzyme, forming a complex with trpE as anthranilate synthase (tryptophan pathway), and with pabB as amino-deoxychorismate (ADC) synthase (folate pathway)"
    • reference:
• Type: Not Target
• Start: 14914
• End: 15612
• Operon:


• Trans-membrane region:
  
• Role: I.G.2 - Folic acid
• GO terms:
  • GO:0016829 - lyase activity (Uniprot)
  • GO:0009073 - aromatic amino acid family biosynthetic process (Uniprot)
  • GO:0008652 - cellular amino acid biosynthetic process (Uniprot)
  • GO:0008152 - metabolic process (Uniprot)
  • GO:0006541 - glutamine metabolic process (Uniprot)
  • GO:0005950 - anthranilate synthase complex (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0004049 - anthranilate synthase activity (Uniprot)
  • GO:0000162 - tryptophan biosynthetic process (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • GLN[c] + CHOR[c] -> GLU[c] + ADCHOR[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6QUR	52%	synthetic construct	Mapping the allosteric communication network of aminodeoxychorismate synthase
  1QDL	43%	Sulfolobus solfataricus	THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS
  1I7S	40%	Serratia marcescens	ANTHRANILATE SYNTHASE FROM SERRATIA MARCESCENS IN COMPLEX WITH ITS END PRODUCT INHIBITOR L-TRYPTOPHAN
  1I7Q	40%	Serratia marcescens	ANTHRANILATE SYNTHASE FROM S. MARCESCENS
  1I1Q	39%	Salmonella typhimurium	STRUCTURE OF THE COOPERATIVE ALLOSTERIC ANTHRANILATE SYNTHASE FROM SALMONELLA TYPHIMURIUM

• Links to additional information on trpG:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -7.258 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology
  • Photo of spot titer

Amino Acid Sequence

MRILVVDNYDSFVFNLVQYLGQLGIEAEVWRNDDHRLSDEAAVAGQFDGVLLSPGPGTPERAGASVSIVHACAAAHTPLLGVCLGHQAIGVAFGATVDRA
PELLHGKTSSVFHTNVGVLQGLPDPFTATRYHSLTILPKSLPAVLRVTARTSSGVIMAVQHTGLPIHGVQFHPESILTEGGHRILANWLTCCGWTQDDTL
VRRLENEVLTAISPHFPTSTASAGEATGRTSA

Additional Information

trpG is a bifunctional enzyme, forming a complex with trpE as anthranilate synthase (tryptophan pathway), and with pabB as amino-deoxychorismate (ADC) synthase (folate pathway)