TB Genome Annotation Portal

Rv0001 (dnaA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: chromosomal replication initiator protein dnaA
  • REFSEQ: chromosomal replication initiation protein
  • PATRIC: Chromosomal replication initiator protein DnaA
  • TUBERCULIST: Chromosomal replication initiator protein DnaA
  • NCBI: Chromosomal replication initiator protein DnaA
  • updated information (H37Rv4):
    • gene name: dnaA
    • function:
    • reference:
• Type: Not Target
• Start: 1
• End: 1524
• Operon:


• Trans-membrane region:
  
• Role: II.A.5 - DNA replication, repair, recombination and restriction/modification
• GO terms:
  • GO:0043565 - sequence-specific DNA binding (Uniprot)
  • GO:0016887 - ATPase activity (Uniprot)
  • GO:0016311 - dephosphorylation (Uniprot)
  • GO:0006275 - regulation of DNA replication (Uniprot)
  • GO:0006270 - DNA replication initiation (Uniprot)
  • GO:0006260 - DNA replication (Uniprot)
  • GO:0006172 - ADP biosynthetic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0003688 - DNA replication origin binding (Uniprot)
  • GO:0003677 - DNA binding (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 3pvv, 3pvp
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  3PVV	100%	Mycobacterium tuberculosis	Structure of Mycobacterium tuberculosis DnaA-DBD in complex with box1 DNA
  3PVP	100%	Mycobacterium tuberculosis	Structure of Mycobacterium tuberculosis DnaA-DBD in complex with box2 DNA
  1J1V	51%	Escherichia coli	Crystal structure of DnaA domainIV complexed with DnaAbox DNA
  2Z4S	38%	Thermotoga maritima	Crystal structure of domain III from the Thermotoga maritima replication initiation protein DnaA
  2Z4R	38%	Thermotoga maritima	Crystal structure of domain III from the Thermotoga maritima replication initiation protein DnaA
  3R8F	36%	Aquifex aeolicus	Replication initiator DnaA bound to AMPPCP and single-stranded DNA
  2HCB	36%	Aquifex aeolicus	Structure of AMPPCP-bound DnaA from Aquifex aeolicus
  1L8Q	36%	Aquifex aeolicus	CRYSTAL STRUCTURE OF DNA REPLICATION INITIATION FACTOR

• Links to additional information on dnaA:
  • Mycobrowser
  • PATRIC
  • KEGG - nucleotide and amino acid sequences of gene
  • BioCyc
  • Systems Biology
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -11.914 (from Jeremy Rock's lab)

Amino Acid Sequence

LTDDPGSGFTTVWNAVVSELNGDPKVDDGPSSDANLSAPLTPQQRAWLNLVQPLTIVEGFALLSVPSSFVQNEIERHLRAPITDALSRRLGHQIQLGVRI
APPATDEADDTTVPPSENPATTSPDTTTDNDEIDDSAAARGDNQHSWPSYFTERPHNTDSATAGVTSLNRRYTFDTFVIGASNRFAHAAALAIAEAPARA
YNPLFIWGESGLGKTHLLHAAGNYAQRLFPGMRVKYVSTEEFTNDFINSLRDDRKVAFKRSYRDVDVLLVDDIQFIEGKEGIQEEFFHTFNTLHNANKQI
VISSDRPPKQLATLEDRLRTRFEWGLITDVQPPELETRIAILRKKAQMERLAVPDDVLELIASSIERNIRELEGALIRVTAFASLNKTPIDKALAEIVLR
DLIADANTMQISAATIMAATAEYFDTTVEELRGPGKTRALAQSRQIAMYLCRELTDLSLPKIGQAFGRDHTTVMYAQRKILSEMAERREVFDHVKELTTR
IRQRSKR

Additional Information